Research Paper Volume 4, Issue 9 pp 636—647

Down regulation of miR-124 in both Werner syndrome DNA helicase mutant mice and mutant Caenorhabditis elegans wrn-1 reveals the importance of this microRNA in accelerated aging

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Figure 3. Reactive oxygen species (ROS) and ATP levels in wild type and mutant C. elegans strains. (A) ROS levels in whole body of 7-day adult old worms (post-larval L4 stage) detected with dichlorofluorescein. Data are expressed as units of fluorescence per micrograms of proteins. (Unpaired Student's t-test; *P = 0.0442 vs. wild type; **P = 0.0008 vs. wild type). (B) ATP levels in whole body of 7-day old adult worms (post-larval L4 stage). (Unpaired Student's t-test; *P = 0.0410 vs. wild type; **P = 0.0137 vs. wild type). Data are expressed as units of fluorescence per micrograms of proteins. Twenty-five worms of each genotype were collected for the ROS or ATP measurements. Experiments were performed with three independent pools of animals.