Research Paper Volume 5, Issue 1 pp 37—50
Specific lipofuscin staining as a novel biomarker to detect replicative and stress-induced senescence. A method applicable in cryo-preserved and archival tissues
Figure 8. Accumulation of lipofuscin in formalin-fixed paraffin-embedded (FFPE) tissues from benign prostatic hyperplasia (BPH) that corresponds to senescent areas as depicted by Senescence-Associated beta-galactosidase (SA-β-gal) in cryo-preserved material. FFPE sections from patients with BPH in enlarged prostates (prostate weight greater than 55gr) demonstrate accumulation of lipofuscin. Sections were deparaffinized and double stained with SBB (dark blue-black granules) and NFR as counterstain (A). Lipofuscin's presence was verified with fluorescence microscopy (B). Immunostaining for Ki-67 shows no matching with lipofuscin accumulation (C). Normal prostate regions adjacent to BPH, were negative for lipofuscin (D). Scale bars: BPH, 100 μm; Normal Prostate, 50 μm. Insets: Cells at higher magnification.