Research Paper Volume 13, Issue 8 pp 12224—12238

Figure 6. EED accelerates the progression of GC in vivo through the miR-338-5p/METTL3/CDCP1 axis. (A) RT-qPCR to examine the expression of EED/miR-338-5p/METTL3/CDCP1 in tumors of nude mice, with β-actin and U6 as internal control, respectively. (B) Immunohistochemistry to detect METTL3 and CDCP1 expression in mouse tumors. (C) Tumor volume and tumor photos in response to EED knockdown and CDCP1 overexpression. (D) Quantitative analysis for tumor weight in response to EED knockdown and CDCP1 overexpression. Measurement data are expressed as mean ± standard deviation. * p < 0.05. Data comparison among multiple groups was performed using one-way ANOVA with Tukey's post hoc test. Data comparison between groups at different time points was performed using two-way ANOVA or repeated-measures ANOVA with Bonferroni post hoc test. n = 6.