Figure 7. Expression of myocardial markers on protein level. (A) The photograph of Western Blot detected the expression of Nkx2.5, GATA4, Mesp1, cTnT, cTnI and α-actin on protein level. (B) Graph of the Western Blot. *P<0.05, **P<0.01, ***P<0.001. Nkx2.5, GATA4, Mesp1, and cTnI were all highly expressed on 13-16 kPa matrix, compared with 62-68kpa and control group. n=3.