Research Paper Volume 13, Issue 2 pp 2941—2958
Up-regulation of miR-204 inhibits proliferation, invasion and apoptosis of gallbladder cancer cells by targeting Notch2
Figure 2. Effects of miR-204 on biological functions of gallbladder carcinoma cells. (A) Expression of miR-204 in GBC-SD, SGC-996, and NOZ cells; (B) Expression of miR-204 in GBC-SD and NOZ cells after transfection with miR-204-mimics, miR-204-inhibit and miR-NC, respectively; (C) Proliferation of GBC-SD and NOZ cells after miR transfection, as detected by CCK-8 assay; (D) Membrane penetration of GBC-SD and NOZ cells after miR transfection by transwell assay; (E) Apoptosis rate of GBC-SD and NOZ cells after transfection by flow cytometry; (F) Migration of GBC-SD and NOZ cells after transfection by cell scratch assay; (G) Expression of Vimentin, N-cadherin and E-cadherin in GBC-SD and NOZ cells by western blot. a indicates in comparison with the miR-NC group, p<0.05.