Research Paper Volume 15, Issue 14 pp 6658—6689
Inhibiting NLRP3 signaling in aging podocytes improves their life- and health-span
Figure 4. Podocyte health was improved upon inhibition/absence of NLRP3. (A) Comparison of the transcripts for canonical podocyte genes (Wt1, Nphs1, Nphs2, Synpo, Cdkn1c, Vegfa) using the normalized mRNA-seq read counts from isolated podocytes of middle-aged saline-treated (red bars) and MCC950-treated mice (blue bars) compared to their young counterparts (grey bars). (B–Z) Immunostaining of kidneys from young, saline- and MCC95-treated middle-aged as well as middle-aged Nlrp3 null mice for WT-1/Wt1 (B–F), Nephrin/Nphs1 (G–K), Podocyin/Nphs2 (L–P), Synaptopodin/Synpo (Q–U) and VEGFA (V–Z). Representative images are shown; scale bars represent 25 μm. Staining intensities were quantified and depicted in the graphs as % tuft area.