Research Paper Volume 15, Issue 16 pp 8220—8236
Inhibition of VCP modulates NF-κB signaling pathway to suppress multiple myeloma cell proliferation and osteoclast differentiation
Figure 1. Elevated VCP is associated with poor survival in MM patients and siVCP inhibits MM cell proliferation in vitro. (A) VCP mRNA levels were significantly increased in MM samples (n = 351) compared with NP (n = 22) and MGUS (n = 44) samples. (B) Higher VCP level was associated with a shorter OS in TT2 cohort (n = 351, High: n = 75, Low: n = 276). (C) Box plot representing VCP expression in eight MM subgroups from TT2 cohort. (D) WB analysis of VCP expression in siVCP cells. (E) MTT assay examined the effect of siVCP on MM cell proliferation. (F) WB analysis showed that siVCP inhibited CDK4/6 expression in MM cells. The MM patient survival data were plotted by Kaplan-Meier curve, and the survival of patients with low and high expression of VCP was compared through a log-rank test. Data are presented as the mean ± SD; *p < 0.05; **p < 0.01; ***p < 0.001.