Research Paper Volume 15, Issue 20 pp 10915—10937

Figure 7. H3K9 acetylation levels mediated by Gcn5 and Hda1 are correlated with senescent populations accumulated in the ageing cell cultures. (A) Global H3K9ac and H3K14ac levels in WT and mutant cells; (B, C) Quantification of H3K9ac (B) and H3K14ac (C); (D) Correlation between H3K9ac levels with CFU/viability ratios. Two-factor ANOVA was performed to reveal the differences between WT and the mutants across different growth phases. ***: p < 0.001, **: 0.001<p< 0.01, *: 0.01<p< 0.05 and n.s.: p> 0.05. Error bars represent standard deviation calculated from biological triplicates. Abbreviation: EXP: exponential phase (6h), PDS: early post-diauxic shift phase (14h) and the transition phase (24h).