Research Paper Volume 15, Issue 21 pp 12209—12224
Effects of amyloid β (Aβ)42 and Gasdermin D on the progression of Alzheimer’s disease in vitro and in vivo through the regulation of astrocyte pyroptosis
Figure 3. siRNA-GSDMD inhibits astrocyte pyroptosis. (A) After the knockdown of GSDMD, astrocytes were stimulated with the 1 μmol Aβ42 for 48 h. All cells were harvested for PI staining, and flow cytometry analysis. *p < 0.05 vs. Aβ42 group. (B) The protein expression of GSDMD in the Aβ42 and siRNA-caspase 1/11 groups was detected by western blotting. (C) Concentrations of TNF-α, IL-1α, IL-1β and IL-18 were measured by enzyme-linked immunosorbent assay. *p < 0.05 vs. Aβ42 group, #p < 0.05 vs. Aβ42 group.