Research Paper Volume 15, Issue 21 pp 12497—12512
Ferulic acid ameliorates the quality of in vitro-aged bovine oocytes by suppressing oxidative stress and apoptosis
Figure 4. FA mitigates aging-induced cellular senescence and DNA damage. (A) Representative fluorescence images of intracellular SA-β-Gal activity in the three groups (Fresh, Aged, and Aged + FA). Scale bar: 100 μm. R = 3. (B) Relative intracellular levels of SA-β-Gal in bovine oocytes from the three groups (Fresh, Aged, and Aged + FA group). (C) Representative fluorescence images of positive and negative γ-H2AX staining. Scale bar: 100 μm. R = 3. (D) The γ-H2AX positivity rate in bovine oocytes from the three groups (Fresh, Aged, and Aged + FA group). *P < 0.05, **P < 0.01, and ***P < 0.001 indicate significant differences.