Research Paper Volume 15, Issue 23 pp 14457—14472

Figure 4. miR-203a-3p inhibited LPS-induced pyroptosis and oxidative stress in chondrocytes. (A–D) Immunofluorescence staining of NLRP3 (green) and GSDMD (red) as well as the relative quantification of positive cells were performed respectively in LPS-induced chondrocytes following transfection with miR-203a-3p mimic, inhibitor and consistent negative controls. (E–G) WB analysis and the relative quantification showing the expression levels of pyroptosis related proteins (NLRP3, ASC, Cleaved caspase-1, GSDMD, I L-1β, and IL-18). (H, I) Malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity in LPS-induced chondrocytes following transfection. (J, K) ROS production in LPS-induced chondrocytes after transfection was measured by DCF staining. All experiments were performed in triplicated and data were presented as the mean±SD, n=3 per group. *P<0.05, **P<0.01.