Research Paper Volume 16, Issue 3 pp 2181—2193

(-)-Epicatechin protects against myocardial ischemia/reperfusion injury via autophagy-dependent ferroptosis

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Figure 5. EPI up-regulates the expression of GFP-LC3 in H9C2 cells and lysosome. (A) Representative fluorescent images of GFP-LC3 and lysosomal co-staining in H9C2 cells. (B) GFP-LC3 dots intensity in H9C2 cells. 2.5-EPI: 2.5 μM EPI; 5-EPI: 5 μM; 10-EPI: 10 μM EPI. Data (n=3) are expressed as mean ± SD, *P < 0.05 compared with OGD/R group, **P < 0.01 compared with OGD/R group.