Research Paper Volume 16, Issue 3 pp 2475—2493

Figure 1. Association of KLF3 down-regulation with prognosis in CRC patients. (A, B) KLF3 expression was significantly lower in 488 CRC specimens compared to 42 adjacent non-CRC specimens, and in matched CRC specimens compared to matched normal colon specimens. The data is presented as (-log10) for KLF3 expression (fpkm). The line shows the average value. (C, D) mRNA and protein levels of KLF3 measured by qPCR and western blotting, respectively, in eight pairs of randomly selected CRC tissues and normal samples. (E) Immunohistochemistry analysis of the expression of KLF3 protein in CRC and normal samples. (F, G) KLF3 mRNA and protein expressions were higher in NCM460 than in normal CRC cell lines. (H, I) Kaplan-Meier curves showing reduced overall survival (OS) and disease-free survival (DFS) in patients with low KLF3 mRNA levels. Scale bars: 50 μm. *p < 0.05; **p < 0.01; ***p < 0.001.