Research Paper Volume 16, Issue 5 pp 4250—4269
Lei’s formula attenuates osteoarthritis mediated by suppression of chondrocyte senescence via the mTOR axis: in vitro and in vivo experiments
Figure 4. LSF inhibits the H2O2-stimulated oxidative stress injury in chondrocytes and modulates the mTOR signaling pathway. (A, B) Gradient concentrations of LSF-containing serum were pre-treated with chondrocytes before being given the appropriate concentration of H2O2 for incubation, and the expression of mTOR was detected by Western blotting experiments. (C, D) qRT-PCR was performed to detect the expression of mTOR and S6 in chondrocytes processed with LSF and H2O2. All data represent mean ± SD. Compared to the control group, ****P < 0.0001; compared to the H2O2-stimulated group, ***P < 0.001, ****P < 0.0001.