Research Paper Volume 16, Issue 5 pp 4541—4562

Figure 2. NTZF can improve the estrous cycle, body weight, serum sex hormone levels and ovarian primordial follicle differentiation. (A) Representative pictures of different estrous cycles (×200 magnification). NEC indicates nucleated epithelial cells, CEC indicates cornified epithelial cells, L indicates leukocytes. (B) Statistical figure of estrous cycle, n = 8. (C) Statistical chart of the difference in body weight of mice before and at the end of the experiment, n = 8. (D) The concentration changes of FSH, LH, E2 and AMH in serum of mice, n = 4. (E) Representative images of HE staining (×400 magnification). Blue arrows indicate primordial follicles, yellow arrows indicate growing follicles, orange arrow indicates mature follicles, and black arrow indicates atretic follicles. (F) Statistics of the ratio of primordial follicles in the ovary of mice. The ratio of primordial follicles = the number of primordial follicles/the total number of follicles ×100%, n = 3. (^*p < 0.05, ^**p < 0.01 versus control group; ^#p < 0.05, ^##p < 0.01, versus model group).