Research Paper Volume 16, Issue 5 pp 4541—4562
Ningxin-Tongyu-Zishen formula alleviates the senescence of granulosa cells on D-galactose-induced premature ovarian insufficiency mice
Figure 5. NTZF protecting OGCs in vitro was associated with Sirt1/p53 signaling pathway. (A–D) The expression of Sirt1 and p53 in each group was detected by IF (×400 magnification). (E–G) The relative mRNA levels of Sirt1, p53, and p21 were analyzed by q-PCR. (H–K) Western blot was used to analyze the protein expression of Sirt1, p53, and p21 in each group (*p < 0.05, **p < 0.01, ***p < 0.001, versus control group, n = 3; #p < 0.05, ##p < 0.01, ###p < 0.001, versus H2O2 group, n = 3).