Research Paper Volume 16, Issue 5 pp 4591—4608

Figure 3. GBA3 enhanced FAO and reduced the intracellular accumulation of lipids. (A) Constructing the cell line overexpressing GBA3. P-value was calculated by 2-tailed Student’s t test (*, P < 0.01). (B) Extracellular acidification rate (ECAR) was analyzed, P-value was calculated by 2-tailed Student’s t test (ns, not significant). (C, D) Respiratory rates expressed as oxygen consumption rate (OCR) were measured using Seahorse metabolic analyzer. P-value was calculated by 2-tailed Student’s t test (***, P < 0.001). (E) Representative images of JC-10 staining (Scale bars, 50 μm). (F) Lipid droplet was visualized by Bodipy 493/503 (green fluorescence), nuclei were counterstained blue with DAPI (blue fluorescence) (Scale bars, 20 μm). (G) Relative levels of free fatty acid, triglyceride and cholesterol were quantified in cells, P-value was calculated by 2-tailed Student’s t test (***, P < 0.001).