Research Paper Volume 16, Issue 5 pp 4811—4831
FHND004 inhibits malignant proliferation of multiple myeloma by targeting PDZ-binding kinase in MAPK pathway
Figure 4. The affinity of FHND004 to PBK protein is better than FHND008. (A) The structure of PBK (PDB: 5J0A). (B, C) The molecular docking of FHND004 and FHND008 with PBK. The green stick in B is FHND004, the red stick in C is FHND008, the PBK is represented by a yellow cartoon, and the hydrogen bond is represented by a dotted line. (D) MST results of FHND004 and FHND008 on PBK wild type. The results showed that the affinity between FHND004 and PBK was better than that of FHND008. (E) MST results after double mutation of N124A and D125A in PBK. The results showed that the affinity of PBK double mutation to FHND004 and FHND008 was significantly decreased.