Research Paper Volume 16, Issue 5 pp 4841—4861

HOXC9 characterizes a suppressive tumor immune microenvironment and integration with multiple immune biomarkers predicts response to PD-1 blockade plus chemotherapy in lung adenocarcinoma

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Figure 3. Confirmation of Hoxc9 knockdown and its impacts on viability and proliferation of CMT167 and LCC cells. (A, B) The knockdown level of Hoxc9 was detected by RT-qPCR. (C, D) The knockdown level of Hoxc9 was detected by western blot. (E, F) The cell viability was significantly impaired under Hoxc9 knockdown. (GI) The influenced proliferation competence was detected with colony formation after 10 days. (*P<0.05, **P<0.01, ***P<0.001).