Compound sophora decoction alleviates ulcerative colitis by regulating macrophage polarization through cGAS inhibition: network pharmacology and experimental validation

Fei Gao; Shuangjiao Deng; Yujin Liu; Pengcheng Wu; Lifen Huang; Feng Zhu; Chunzhu Wei; Yuyi Yuan; Yang Gui; Yushi Tian; Heng Fan; Hui Wu

doi:doi:10.18632/aging.205734

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Research Paper Volume 16, Issue 8 pp 6921—6936

Compound sophora decoction alleviates ulcerative colitis by regulating macrophage polarization through cGAS inhibition: network pharmacology and experimental validation

Figure 4. CSD inhibits cGAS in macrophages (RAW264.7) and regulates cellular inflammation in vitro. (A) Representative western blot of cGAS; (B) Statistical map of cGAS protein content; (C) cGAS mRNA levels; (D) IFN-β mRNA levels; (E) CCL17 mRNA levels; (F) IL10 mRNA levels; (G) TNF-α mRNA levels; (H) CXCL10 mRNA levels. The data present the mean ± SD of three independent experiments. Abbreviation: ns: no significance. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs. ISD group.