Research Paper Volume 16, Issue 8 pp 7217—7248

Figure 3. Hepatic METTL3 homozygous knockout by Alb-iCre mice (GPT) induces abnormal lipid accumulation in mouse hepatocytes. (A–C) Representative H&E staining photographs of liver sections from control mice and METTL3^Δhep mice (GPT) at 4 (A), 7 (B) and 14 (C) days postnatally. Scale bar = 100 μm. (D) Heatmap depicts the differential expression of hepatic lipid metabolism-related genes from RNA-seq results deposited in NCBI GEO under the accession number GSE198512 [17]. In the cluster heatmap, class comparison and hierarchical clustering of differentially expressed genes (DEGs) involved in hepatic lipid metabolism were performed between control mice and METTL3^Δhep mice (GPT) at 2 weeks after birth. Genes with increased and reduced expressions are shown in red and blue, respectively. (E) GO analysis of up- and downregulated genes related with hepatic lipid metabolism (from RNA-seq data deposited in GEO under accession number GSE198512) in the liver of control mice and METTL3^Δhep mice (GPT) at 2 weeks after birth. (F) qRT-PCR analysis of the mRNA expression of genes related with to fatty acid oxidation, cholesterol efflux, lipid metabolic process, lipid transport and lipid biosynthetic process in the livers of METTL3^Δhep mice (GPT).