Research Paper Volume 16, Issue 8 pp 7474—7486

Figure 7. X5 inhibited autophagy-induced injury by H₂O₂ in PC12 cells. (A–C) X5 inhibited the expression of autophagy protein. PC12 cells were pre-incubated with different concentrations of X5 (1, 5 and 10 μM) and CUR (5 μM) for 18 h, then stimulated with H₂O₂ (800 μM) for 12 h. The protein expression of Beclin-1 and LC3-β was detected by Western blot. (D, E) X5 increased the expression of SIRT1 protein. PC12 cells were incubated with X5 (1, 5 and 10 μM) and CUR (5 μM) for 18 h, and the expression of SIRT1 protein was detected by Western blot. (F) SIRT1 plays an important role in H₂O₂-induced injury. SIRT1 was silenced in PC12 cells with siRNA, PC12 cells were incubated with X5 (1.25 μM) for 18 h, treated with H₂O₂ (600 μM) for 24 h, and cell viability was measured by MTT assay. The data are expressed as mean ± SD, n ≥3. ^####p<0.0001, ^#p<0.05 vs DMSO, ^****p<0.0001, ^***p<0.001, ^**p<0.01, ^*p<0.05 vs H₂O₂.