Research Paper Volume 16, Issue 8 pp 7311—7330
EIF3B stabilizes PCNA by counteracting SYVN1-mediated ubiquitination to serve as a promotor in cholangiocarcinoma
Figure 2. EIF3B depletion suppressed cholangiocarcinoma development in vitro. (A) The cell proliferation abilities of HCCC-9810 and RBE cells after being transfected shEIF3B or shCtrl were assessed by MTT assay. (B) After being transfected shEIF3B or shCtrl, the capacities of HCCC-9810 and RBE cells to form colony were detected. (C, D) After being transfected shEIF3B or shCtrl, the changes of cell migration of HCCC-9810 and RBE cells were evaluated by transwell assay (C) and wound-healing assay (D). Scale bar: 50 μm for transwell assay and 200 μm for wound-healing assay. Magnification times: 200 × for transwell assay and 50 × for wound-healing assay. (E) The effects of EIF3B knockdown on cell apoptosis of HCCC-9810 and RBE cells were examined by flow cytometry. (F) The levels of apoptosis-related proteins in HCCC-9810 and RBE cells transfected with shEIF3B and shCtrl were measured by western blot. All the experiments were in triplicate. Results were presented as mean ± SD. ** P < 0.01, *** P < 0.001.