Research Paper Volume 16, Issue 9 pp 7818—7844

Figure 16. Validation of the role of IGFBP1 in the unfolded protein response of gastric cancer. (A) MKN45 and AGS cells were transfected with siIGFBP1 and overexpressed IGFBP1 in HGC-27 cells. (B) Protein expression changes of CHOP, GRP78, XBP1 after IGFBP1 supplementation in MKN45 cells. (C) Verify knock down efficiency of shIGFBP1. (D) Anatomical images of lung metastases 4 weeks after caudal vein inoculation with gastric cancer cells. Lung metastasis was inhibited in cancer cells transfected with shIGFBP1 compared to shNC. (E) Statistical histogram of pulmonary metastatic nodules. (F) H&E staining in the pathology of lung metastases. (G) Images of xenografts dissected from nude mice 5 weeks after subcutaneous injection. (H) Graph showing the average tumor weight of the resected tumor. (I) Tumor volume curve showing tumor growth. (J) Representative IHC images of the shIGFBP1 and shNC were differentially expressed CHOP, GRP78, XBP1, Ki67 and Cleaved Caspase 3. *P < 0.05, **P < 0.01.