Research Paper Volume 16, Issue 9 pp 7523—7534

Figure 2. Inhibition of DNA synthesis in H₂O₂-induced BJ and T47D. Cells were treated with H₂O₂ to induce senescence. (A) Detection of EdU incorporated into the DNA of senescent and non-senescent fibroblasts and cancer cells treated or not with WEW. DNA synthesis was evaluated by immunofluorescence staining using EdU, and its incorporation was detected with Alexa Fluor 488 (green). Counterstaining with DAPI displays the localization of the nuclei (blue). (B) The results are expressed as a percentage of EdU incorporation after oxidative stress senescence induction and treatment with WEW or tap water. Results are given as mean ±SEM of five different fields, and significance was calculated by a two-tailed Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001; ns: non-significance.