Research Paper Volume 16, Issue 9 pp 7523—7534

Figure 3. WEW significantly reduces the expression of p21 but does not affect proliferation of BJ and T47D cells. (A) Relative mRNA level of the p21 gene in BJ senescent cells and T47D senescent cells treated with WEW compared to senescent cells treated with tap water. (B) Protein level expression of p21 determined by western blot analysis (top panel: picture of the blots; bottom panel: quantification). Tubulin was used as internal loading control. (C) Relative mRNA level of the Ki67 gene in BJ senescent cells and T47D senescent cells treated with WEW compared to senescent cells treated with tap water. The graphs represent the mean ±SEM from three independent experiments. Significance was calculated by a two-tailed Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001.