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Primer sequences and probes used for Taqman PCR:
mmu HIF-1( upper, 5-gcactagacaaagttcacctgaga-3
mmu HIF-1( lower, 5-cgctatccacatcaaagcaa-3, Probe: #95
mmu PDK-1 upper, 5-gttgaaacgtcccgtgct-3
mmu PDK-1 lower, 5-gcgtgatatgggcaatcc-3, Probe: #20
mmu GLUT-1 upper, 5-atggatcccagcagcaag-3
mmu GLUT-1 lower, 5-ccagtgttatagccgaactgc-3, Probe: #52
mmu PFK-L upper, 5-ccatggacgaggagaggtt-3
mmu PFK-L lower, 5-tccagttgttctcaaagctcct-3, Probe: #42
mmu ENO-1 upper, 5-gaggcgcttagtgctgct-3
mmu ENO-1 lower, 5-agaatagacatggcgaatttctg-3, Probe: #91
mmu OSM-R upper, 5- ccaaaaagagttcagcacacc -3
mmu OSM-R lower, 5- ccgaccacacttgtctccat -3, Probe: #32
mmu PDGFRa upper, 5-gtcgttgacctgcagtgga-3
mmu PDGFRa lower, 5-ccagcatggtgatacctttgt-3, Probe: #80
mmu SOCS-3 upper, 5-atttcgcttcgggactagc-3
mmu SOCS-3 lower, 5-aacttgctgtgggtgaccat-3, Probe: #83
mmu ATP-5L upper, 5-aaggaagctgtgctgaatgg-3
mmu ATP-5L lower, 5-atgccacgtttgcctatga-3, Probe: #42
mmu FH-1 upper, 5-gcaccccaatgatcatgtta-3
mmu FH-1 lower, 5-cattgctgtgggaaaggtg -3, Probe: #106
mmu NDUFB4 upper, 5-agggtggtgaagtggaagag-3
mmu NDUFB4 lower, 5-tggccacttccactggtta-3, Probe: #31
mmu NDUFA6 upper, 5-cccactcccaagaactcact-3
mmu NDUFA6 lower, 5-tggataagtctttcctttctgtcc-3, Probe: #17
hsa HIF-1( upper, 5-ggttcactttttcaagcagtagg-3
hsa HIF-1( lower, 5-tggtaatccactttcatccattg-3, Probe: #3
hsa PDK-1 upper, 5-gctgggtaatgaggatttgact-3
hsa PDK-1 lower, 5-aagtctgtcaattttcctcaaagg-3, Probe: #10
hsa ENO-1 upper, 5-gctccgggacaatgataaga-3
hsa ENO-1 lower, 5-tgatgtgctcaacagccttt-3, Probe: #60
Antibodies. Rabbit polyclonal against p-Stat3, p-Akt and Caspase-3 (Cell Signaling Technology, Danvers MA, USA), Stat3 and Actin (Santa Cruz Biotechnology, Santa Cruz CA, USA), Vdac-1 (Abcam, Cambridge, UK). Mouse monoclonal against Hif-1( (Novus Biologicals, Littleton CO, USA), Sod2 (Abcam) and (-Tubulin (Santa Cruz Biotechnology). Mouse monoclonal against Akt was generated in house by immunization with a MBP-Akt fusion protein and purified against the recombinant protein. The MitoProfile total OXPHOS rodent WB Antibody Cocktail (Mitosciences, Eugene OR, USA) was used for mitochondrial complexes.
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