%A Cao, Wenpeng 
%A Chen, Xiaozhong 
%A Xiao, Chaolun 
%A Lin, Dengxiao 
%A Li, Yumei 
%A Luo, Shipeng 
%A Zeng, Zhirui 
%A Sun, Baofei 
%A Lei, Shan 
%D 2023
%T Ar-turmerone inhibits the proliferation and mobility of glioma by downregulating cathepsin B
%! Ar-turmerone inhibits the proliferation and mobility of glioma by downregulating cathepsin B
%K ar-turmerone, glioma, cathepsin B, proliferation, mobility, cell cycle
%X Ar-turmerone, a compound isolated from turmeric seeds, has exhibited anti-malignant, anti-aging and anti-inflammatory properties. Here, we assessed the effects of ar-turmerone on glioma cells. U251, U87 and LN229 glioma cell lines were treated with different concentrations of ar-turmerone (0, 50, 100 and 200 μM), and their viability and mobility were evaluated using Cell Counting Kit 8, colony formation, wound healing and Transwell assays. The effects of ar-turmerone on U251 glioma cell proliferation were also assessed using a subcutaneous implantation tumor model. High-throughput sequencing, bioinformatic analyses and quantitative real-time polymerase chain reactions were used to identify the key signaling pathways and targets of ar-turmerone. Ar-turmerone reduced the proliferation rate and mobility of glioma cells in vitro and arrested cell division at G1/S phase. Cathepsin B was identified as a key target of ar-turmerone in glioma cells. Ar-turmerone treatment reduced cathepsin B expression and inhibited the cleavage of its target protein P27 in glioma cells. On the other hand, cathepsin B overexpression reversed the inhibitory effects of ar-turmerone on glioma cell proliferation, mobility progression in vitro and in vivo. In conclusion, ar-turmerone suppressed cathepsin B expression and P27 cleavage, thereby inhibiting the proliferation and mobility of glioma cells.
%U https://doi.org/10.18632/aging.204940
%J Aging
%0 Journal Article
%V 15
%N 18
%P 9377-9390
%R 10.18632/aging.204940
%@ 1945-4589