%A Hu, Qihao 
%A Chen, Shi 
%A Li, Yukun 
%A Hu, Teng 
%A Hu, Jianpeng 
%A Wang, Cheng 
%A Yang, Fei 
%A Yang, Xiang 
%A Zhou, Feng 
%A Liu, Zhengdong 
%A Xu, Wei 
%A Zhang, Ji 
%D 2023
%T ANGPTL4, a direct target of hsa-miR-133a-3p, accelerates lung adenocarcinoma lipid metabolism, proliferation and invasion
%! ANGPTL4, a direct target of hsa-miR-133a-3p, accelerates lung adenocarcinoma lipid metabolism, proliferation and invasion
%K ANGPTL4, lung adenocarcinoma, hsa-miR-133a-3p, lipid metabolism reprogramming, bioinformatics
%X Background: Globally, lung adenocarcinoma (LUAD) is the most common type of lung cancer. The secreted protein angiopoietin-like 4 (ANGPTL4) has been implicated in a number of physiological and pathological processes, including angiogenesis and lipid metabolism. But the role of ANGPTL4 in LUAD remains unknown.
Methods: The expression of ANGPTL4 and miR-133a-3p was confirmed by public database analysis. Xenograft model, MTT, Clone formation and EdU analysis were used to confirm the effects of miR-133a-3p/ANGPTL4 on LUAD cell proliferation and growth. Wound healing and Transwell analysis were used to elucidate the role of miR-133a-3p/ANGPTL4 in LUAD cell migration and invasion. Oil red O staining was used to confirm ANGPTL4 in LUAD lipids production. Dual-luciferase reporter gene analysis was used to demonstrate miR-133a-3p could directly bind ANGPTL4 3′-UTR. WB and PCR were used to confirm the protein expression of ANGPTL4.
Results: ANGPTL4 was significantly increased in LUAD samples, which could promote LUAD cell proliferation, migration, invasion, growth and lipid production. miR-133a-3p could directly bind to ANGPTL4 mRNA, and repress the expression ANGPTL4, resulting in suppressing LUAD proliferation and metastasis.
Conclusion: In conclusion, miR-133a-3p/ANGPTL4 axis might be a potential biomarker and therapeutic target for LUAD patients.
%U https://doi.org/10.18632/aging.205313
%J Aging
%0 Journal Article
%V 16
%N 9
%P 8348-8360
%R 10.18632/aging.205313
%@ 1945-4589