Perspective Volume 1, Issue 2 pp 146—153

Figure 1. CD3 expression on DN thymocytes shows an age-dependent increase. Thymocytes from different aged mice were stained with anti-CD3, anti-CD4 and anti-CD8 mAb, analysed by flow cytometry and CD3 on DN cells was determined gating the appropriate population. This study revealed that the proportion of CD3⁺ DN thymocytes showed an age-dependent increase. (One month n=5; six months n=5; 12 months n=8; 18 months n=4). *P<0.05; **P<0.01; ***P<0.001.