Research Paper Volume 1, Issue 3 pp 316—327

Oncogenic viral protein HPV E7 up-regulates the SIRT1 longevity protein in human cervical cancer cells

Figure 2. HPV E7 maintains survivin protein and induces site-specific changes in histone H3 modifications.. (A) Histone H3 modifications at 24, 48 and 72 h post-transfection with E6 or E7 siRNA. Equivalent exposures for each pair of immunoblots shown. (B) Aurora B and survivin protein levels following E6 or E7 silencing. (C) Cell death after E6 or E7 siRNA treatment. (D) Relative changes in S10P H3, survivin, p53 and p21 proteins in response to E7 or E6 siRNA. (E) RT-PCR determinations of survivin and lamin A/C mRNA levels in SiHa cells as indicated. (F) Immunoblots showing effect of aurora B silencing on survivin protein levels at 48h post siRNA transfection.