Research Paper Volume 1, Issue 3 pp 316—327

Oncogenic viral protein HPV E7 up-regulates the SIRT1 longevity protein in human cervical cancer cells

Figure 3. Effects of RNAi-mediated silencing of survivin and aurora B on S10P histone H3 and SiHa cell phenotype.. (A) Survivin, aurora B and S10P histone H3 protein levels 48h post-transfection. (B) Phase contrast images of SiHa cells as indicated. (C) Cell cycle distribution at 0, 24, 48 and 72h post-transfection with indicated siRNAs. (D) Induction of polyploidy in SiHa cells following survivin depletion indicated by percentage of >G2 cells and appearance of multinuclear cells.