Research Paper Volume 1, Issue 3 pp 328—334

The S-adenosylmethionine dependent O-methyltransferase PaMTH1: a longevity assurance factor protecting Podospora anserina against oxidative stress


Figure 2. Growth rates of wild-type strain s and of the PaMth1 deletion strain on synthetic PASM medium containing different amounts of CuSO4 and of hydrogen peroxide, respectively. Pairs of strains (wild-type strain s and the PaMth1 knock-out strain) were grown on one plate and growth rates were recorded over 3 days. Cultures of the PaMth1 deletion strain (n = 20) were characterised by decreased growth rates compared to the wild-type strain s (n = 20) when incubated on medium containing 40 μM (p = 6.154e-08) or 80 μM (p = 1.921e-08) CuSO4 and 0.01% (p = 1.443e-08); 0.02% (p = 1.06e-07) or 0.04% (p = 0.99) hydrogen peroxide. Plates containing hydrogen peroxide were incubated in the dark.