Priority Research Paper Volume 1, Issue 8 pp 699—713

Figure 1. Constitutive UCP expression in adult IPCs results in decreased steady state intracellular Ca ²⁺ levels. (A) Under normal growth conditions, the Cg-2 protein in the adult IPCs produces fluorescent signals reflecting a steady state Ca²⁺ flux whereas a 21-51% decrease in average fluorescent intensity is detected in adult IPCs of two dilp2-Gal4/UAS-mucp1, UAS-cg-2 co-expressing fly lines (mUCP1-1 and mUCP1-2), consistent with attenuated insulin action. Each bar represents mean + S.E.M. *p=0.0004, **p<0.0001 (Student's t-test). Shown are average fluorescent signals from IPCs of 10 brains of each line in a representative experiment (see Materials and Methods). Reproducible results were obtained in three independent experiments. Representative images of IPCs from a control adult brain isolated from a dilp2-Gal4/UAS-cg-2 fly line (B) and an adult brain isolated from a dilp2-Gal4/UAS-mucp1, UAS-cg-2 fly line (C) are shown. Images were taken with a 40X objective. Scale bar, 100 μm.