Research Paper Volume 1, Issue 12 pp 1008—1016
Quantifying pharmacologic suppression of cellular senescence: prevention of cellular hypertrophy versus preservation of proliferative potential
Figure 10. Visualization of loss of hypertrophy during proliferation of competent cells. 500 HT-p21 cells (A) were treated with IPTG (B) or IPTG plus rapamycin (C), as indicated, or left untreated. After 3 days, plates were washed and incubated without drugs to allow proliferation. (A) Normal size of proliferating cells. (B) Cellular hypertrophy of senescent cells. (C) Example 1. Clonal proliferation of competent cells results in loss of hypertrophy. (C) Example 2. Cells that remained arrested remained hypertrophic.