Research Paper Volume 1, Issue 12 pp 1008—1016
Quantifying pharmacologic suppression of cellular senescence: prevention of cellular hypertrophy versus preservation of proliferative potential
Figure 6. Clonal proliferation of competent cells. HT-p16 cells were plated in 100-mm plates. The next day, 50 μM IPTG with or without rapamycin, if indicated (RAPA), was added. After 3 days, the plates were washed to remove IPTG and RAPA. (A) Photographs. Upper panel: On days 5 and 8 (after IPTG removal), plates were fixed, stained and photographed. Lower panel: On days 5 and 8 (after IPTG removal), plates were fixed, stained and photographed. (B) Number of colonies. On days 6, 7, 8 and 9 (after IPTG removal), plates were fixed, stained and photographed. The number of colonies was counted and results are shown as percent of plated cells in log-scale.