Research Paper Volume 1, Issue 12 pp 1008—1016

Quantifying pharmacologic suppression of cellular senescence: prevention of cellular hypertrophy versus preservation of proliferative potential

class="figure-viewer-img"

Figure 7. The dynamics of cell numbers. 500 HT-p21 cells were plated in 12 well plates. On the next day, either IPTG alone (I) or IPTG plus rapamycin (I+R) were added. After 3 days, plates were washed (I/w and I+R/w) or left unwashed. Cells were counted at days 1, 3, 6 and 9. Upper panel: linear-scale. Lower panel: log-scale. Open and closed squares: IPTG and IPTG plus Rapa, respectively. Open and closed circles: IPTG washed (I/w) and IPTG plus Rapa washed (I+R/w), respectively. In the presence of IPTG (open squares) and IPTG plus rapamycin (closed squares), the cells did not proliferate.