Research Paper Volume 2, Issue 6 pp 344—352
The choice between p53-induced senescence and quiescence is determined in part by the mTOR pathway
Figure 2. Depletion of TSC2 converts quiescence into senescence in WI-38tert cells. (A)Immunoblot. WI-38tert cells were transduced with shTSC or control pLKO and cultured for 5 days. (B) WI-38tert cells were transduced with lentiviruses. Next day, medium was replaced and Nutlin (10 uM) with our without rapamycin was added. After 4 days cells were washed and stained for beta-Gal. Bars 50 um. (C) WI-38tert cells were transduced with lentiviruses. Next day, medium was replaced and Nutlin (10 uM) was added. After 4 days cells were washed and counted after 6 days.