Research Paper Volume 2, Issue 9 pp 567—581

Klotho interferes with a novel FGF-signalling pathway and insulin/Igf-like signalling to improve longevity and stress resistance in Caenorhabditis elegans

Figure 1. Characterization of Klotho in C. elegans.. (A) Genomic organization of both C. elegans C50F7.10 (1,95 kb) and E02H9.5 (2,3 kb) genes, localized on chromosome IV and III, respectively. Coding regions are indicated by boxes, and introns are represented as lines. The corresponding ORFs share similar size (about 1,44 kb) and are organized in 8 and 7 exons for C50F7.10 and E02H9.5, respectively. (B) The predictive molecular organization of either C50F7.10 or E02H9.5 gene products essentially consists in a sole b-glucosidase-like KL1 domain. Note that a KL1 form of Klotho may be expressed either by differential splicing or post- translational cleavage, in mammals. (C) Alignment of alternatively spliced forms of human and mouse Klotho compared to both C. elegans C50F7.10 and E02H9.5 gene products, identified in the WormBase bank as WP: CE 04248 and WP: CE 09122, respectively. Identical amino acid residues are highlighted. The conserved KL1 domain is underlined. Alignment was performed using the ESPript program [60].