Figure 3. Klotho gene knockdown cannot suppress the Clr phenotype induced by genetic activation of EGL-15.. Adult worms were pre-induced with either klotho or control RNAi at permissive temperature (15°C), prior to being either maintained at 15°C or shifted to restrictive temperature (25°C) which allows the development of a Clr phenotype. (A) Experiments were conducted in a clr-1(e1745) genetic background. The induced Clr phenotype is characterized by both intestine and gonad floating in an enlarged fluid-filled pseudocoelomic cavity. (B) Controls, performed in a clr-1(e1745); let-756(s2613) reduction-of-function double mutant show a partial suppression of Clr phenotype, independent of the klotho gene status.