Research Paper Volume 2, Issue 9 pp 582—596

ATM-independent, high-fidelity nonhomologous end joining predominates in human embryonic stem cells


Figure 7. High-fidelity NHEJ decreases through differentiation. BG01V/-, NP/-, and astrocyte/NHEJ-red cells were infected with Ad-SceI and collected 24 h after infection. DNA was amplified with Amplitaq Gold and was digested with PsiI endonuclease where indicated. (Columns) High-fidelity NHEJ levels were determined by the relative level of the digested portion (PsiI-sensitive) of the PCR DNA fragment as a fraction of uncut DNA; (Error bars) SEM for three samples. Fold (x) indicates changes in relative repair levels when compared to the astrocyte samples.