Research Paper Volume 2, Issue 12 pp 990—998

Figure 1. Effect of statn on chondrocyte aging in vitro.. Chondrocyte senescence was measured by the senescence-associated β-galactosidase (SA-β-Gal) activity assay. The chondrocytes were cultured with IL-1β (10 ng/ml) in the presence or absence of statin (1.0, 10.0 μM) for 5 days. (A-F) Representative images of SA-β-Gal staining, (A) control (medium only), (B) IL-1β-treated group, (C) statin (1.0 μM)-treated group, (D) IL-1β + sratin (1.0 μM)-treated group, (E) statin (10.0 μM)-treated group, (F) IL-1β + sratin (10.0 μM)-treated group. As shown in the representative images, whereas IL-1β stimulated the expression of SA-β-Gal in chondrocytes, treatment with statin markedly inhibited the expression of SA-β-Gal even in the presence of IL-1β stimulation. (G) Treatment with statin significantly inhibited the IL-1β-enhanced SA-β-Gal activity in the chondrocytes.