Research Paper Volume 2, Issue 11 pp 791—803

Figure 1. Inhibition of vessel formation by hUCBSC treatment. Conditioned medium was collected from the single and co-cultures (with hUCBSC) of glioma cells (SNB19, U251 and 5310) and added to HMEC cells pre-seeded (5000 cells/well) in 96-well plates. After 72h of incubation, HMEC cells were observed for capillary-like network formation and photographed under a light microscope. Bar = 500μm. Similarly, conditioned medium was collected from the glioma cells treated either with FAK inhibitor (10μM for 1.5h) or transfected with siFAK. Conditioned medium was added to HMEC cells pre-seeded (5000 cells/well) in 96-well plates. After 72h of incubation, HMEC cells were observed for capillary-like network formation and photographed under a light microscope.