Research Paper Volume 2, Issue 12 pp 914—923

Regulation of the mPTP by SIRT3-mediated deacetylation of CypD at lysine 166 suppresses age-related cardiac hypertrophy


Figure 2. Cyclophilin D is acetylated at K166. (A) Representative m/z spectrum of peptide TDWLDG-AcK-HVVFGHVK obtained from mass spectrometry analyses of Flag-purified mouse Cyclophilin D. (B) Sequence of Cyclophilin D (Mus musculus). Location of the identified acetylation site. (Peptide: blue; acetylated Lysine: red). (C) Protein sequence alignment of CypD from Homo sapiens, Mus musculus, Danio rerio, Saccharomyces cerevisiae. Lysine 166 is marked in red.