Research Paper Volume 2, Issue 12 pp 914—923

Regulation of the mPTP by SIRT3-mediated deacetylation of CypD at lysine 166 suppresses age-related cardiac hypertrophy


Figure 4. Age- and stress-dependent development of cardiac hypertrophy in SIRT3 −/− mice. (A) Kaplan-Meier survival plot of 3 months old wt and SIRT3−/− mice after the Transverse Aortic Constriction (TAC) surgery. wt (n=8), SIRT3−/− (n=11). p < 0.05. (B) Representative image and Masson's trichrome staining of hearts from wt and SIRT3−/− mice 4 weeks after the TAC surgery. Heart weight tibia length ratio of wt and SIRT3−/− mice 30 days after the TAC surgery wt (n=8), SIRT3−/− (n=4) mice. (C) Interventricular septal thickness at diastole (Ivs;d) of 2 and 13 month old wt and SIRT3−/− mice after transverse aortic constriction (TAC) for 4 weeks. (D) Representative images of Masson's trichrome staining of transverse sections of heart from 13 month old wt and SIRT3−/− mice. Collagen deposits (fibrosis) stain blue. n=4 mice per group and age (magnification, 20x). **p<0.01. *** p < 0.001. All error bars represent s.e.m.