Research Paper Volume 2, Issue 12 pp 999—1003

Systemic signals in aged males exert potent rejuvenating effects on the ovarian follicle reserve in mammalian females

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Figure 2. Ovarian Stra8 expression is increased by exposure to blood of aged males. (A) Analysis of Dazl, Stra8 and β-actin mRNA levels in ovaries of YF mice 5 weeks after parabiosis with YF, YM or AM mice (n = 4 mice per group, with results from each mouse shown; no RT, RNA without reverse transcriptase, used as a negative control). (B, C) Examples of STRA8-positive cells in ovaries of YF mice 5 weeks after parabiotic joining with AM mice, as detected by immunofluorescence (red immunopositive signal against blue DAPI counterstain).