Research Paper Volume 3, Issue 2 pp 125—147

Modulation of lipid biosynthesis contributes to stress resistance and longevity of C. elegans mutants


Figure 1. Survivals on agar of wild-type and near-isogenic mutant strains. Worm cohorts were syn-chronized by recovery of eggs after lysis of gravid hermaphro-dites in alkaline hypochlorite (see Materials and Methods), and then by manual selection of L4 larvae. Groups of 25 adults (50 total per group) were transferred to fresh plates daily until no further progeny were produced, and on alternate days thereafter. The x-axis indicates adult age in days, thus excluding effects of slowed development. A illustrates examples of replicate experiments, all of which produced similar sets of survival curves. B shows survival data for N2DRM, daf-2(e1370) and age-1(mg44)-F2 worms, combined with earlier data (dark green triangles and light blue circles, redrawn from [7].