Research Paper Volume 3, Issue 2 pp 125—147

Modulation of lipid biosynthesis contributes to stress resistance and longevity of C. elegans mutants


Figure 3. Steady-state transcript levels for genes of lipid metabolism. Real-Time Polymerase Chain Reaction (RT-PCR) was performed for the six indicated groups of C. elegans (see legend panel). In the experiment shown, the number of independent biological preparations was as follows (groups as displayed from left to right): N2DRM adults, N=8; SR807 [age-1(hx546)], N=3; SR808 [age-1(mg44)], first (F1) homozygous generation,N=3; SR808 [age-1(mg44)], F2 generation,N=4; N2DRM dauer larvae, N=2; and SR806 [daf-2(e1370)], N=3. The ordinate (y axis) displays the base-2 logarithm of transcript level, after normalization to N2DRM controls (which thus always have a mean log2 value of zero). The y value is equivalent to - [tc(mutant) - tc(N2)], where tc is the cycle number required to achieve an arbitrary threshold level of PCR amplification product. P values of <0.1 (based on single-tailed, heteroscedastic t tests) are indicated above or below a histogram bar to indicate a comparison of that strain to N2 controls, or above a bracket to indicate a comparison between the two strains linked by the bracket. This experiment was performed three times with similar results.

A, elo-1 (encoding an FA elongase); B, elo-2 (elongase); C, elo-5 (branched‐chain FA elongase); D, fat-4 (Δ5 desaturase); E, fat‐6 (Δ9 desaturase); F, fat‐7 (Δ9 desaturase).