Research Paper Volume 3, Issue 3 pp 223—236

Gain of survival signaling by down-regulation of three key miRNAs in brain of calorie-restricted mice


Figure 5. Altered expression of active apoptosome-specific proteins in calorie-restricted mouse brain tissue and in over-expressed miRNA cell lines. Cleaved Caspase-9 (C-Caspase-9) and cleaved Caspase-3 (C-Caspase-3) are important components of the apoptosome complex. (A) In calorie-restricted (CR)-fed mouse brain tissues, levels of c-Caspase-9 and -3 decline in an age-dependent manner, compared to Ad Lib (AL)-fed mice. (B) Over-expression of three miRNAs in HEK-293 and NIH/3t3 cells produces a significant increase in cleaved form as compared to scrambled and vector alone controls. Increase in these apoptosome-specific proteins indicates the pro-apoptotic effect of these miRNAs. (**p<0.01, **p<0.0001; all histograms represent average ±