Research Paper Volume 3, Issue 4 pp 380—390

Generation of induced pluripotent stem cell lines from 3 distinct laminopathies bearing heterogeneous mutations in lamin A/C


Figure 2. iPSCs with mutations in lamin A/C display human ESC-characteristics and an intact nuclear membrane. (A) Immunofluorescence photographs for the indicated ESC markers in iPSC clones derived from DCM, aWS, and HGPS patients; iPSCs derived from normal fibroblasts were used as control. Phase contrast photographs and AP staining captures are also included. Scale bars indicate 400 μm. (B) qPCR for endogenous ESC transcription factors in similar iPSC clones. Values are referred to control fibroblasts; H9 ESCs and non-affectediPSCs were also added (also in C). (C) qPCR for lamin A/C mRNA in similar iPSC clones and the respective donor cells.